高铜日粮对肉鸡肾氧化损伤和Nrf2信号通路相关基因表达的影响

doi:10.11843/j.issn.0366-6964.2019.09.020

Abstract

Abstract: In order to investigate the effect of high dietary copper on oxidative damage and expression of Nrf2 signaling pathway related genes in the kidneys of broilers, 48 one-day-old healthy white feather broilers were randomly divided into 4 groups with 12 each in this study. The birds were fed with basal diets (Cu 11 mg·kg^-1, the control group), and high-copper diets (Cu 110 mg·kg^-1, the high-copper group Ⅰ; Cu 220 mg·kg^-1, the high-copper group Ⅱ; Cu 330 mg·kg^-1, the high-copper group Ⅲ, respectively). On day 49, the kidneys were collected for histopathology examination. The activities of superoxide dismutase (SOD), glutathione reductase (GR), and total antioxidant capacity (T-AOC); and the content of malondialdehyde (MDA) were detected. Additionally, mRNA levels of nuclear factor E2-related factor 2 (Nrf2), glutamate cysteine ligase catalytic subunit (GCLC), glutamate cysteine ligase modifier subunit (GCLM), hemeoxygenase-1 (HO-1), and NAD (P) H quinone oxidoreducetase 1 (NQO1) were measured using real-time quantitative PCR (RT-qPCR). The results showed that widen renal interstitial and exfoliated renal tubular epithelial cells were observed in the high-copper groups. Additionally, the activities of SOD, GR, and T-AOC in the high-copper group Ⅲ were significantly decreased compared to those in the control group, but there was no significant change in the content of MDA. Moreover, mRNA levels of Nrf2 and GCLC in the high-copper group Ⅱ and the high-copper group Ⅲ were markedly higher than those in the control group, and mRNA levels of GCLM and HO-1 up-regulated with the increase of dietary copper, but NQO1 mRNA level did not significantly changed. The results suggest that high dietary copper can induce oxidative damage and the expression of Nrf2 signaling pathway related genes in the kidneys of broilers.

CLC Number:

S856.9

PEI Ruonan, YANG Fan, LIAO Jianzhao, MA Feiyang, MA Xinyan, LIN Yuyin, YAO Qifa, TANG Zhaoxin, LIANG Zhaoping. The Effect of High Dietary Copper on Oxidative Damage and Expression of Nrf2 Signaling Pathway Related Genes in the Kidneys of Broilers[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(9): 1912-1919.

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References

[1] GAETKE L M, CHOW C K. Copper toxicity, oxidative stress, and antioxidant nutrients[J]. Toxicology, 2003, 189(1-2):147-163.
[2] VÉLEZ-ALAVEZ M, LABRADA-MARTAGÍN V, MÉNDEZ-RODRIGUEZ L C, et al. Oxidative stress indicators and trace element concentrations in tissues of mako shark (Isurus oxyrinchus)[J]. Comp Biochem Physiol A Mol Integr Physiol, 2013, 165(4):508-514.
[3] 崔伟,李敏,彭西,等. 高铜对雏鸡肾脏组织结构及生化指标影响的研究[J]. 畜牧兽医学报, 2010, 41(1):86-91.
CUI W, LI M, PENG X, et al. Effect of dietary high copper on tissue structure and biochemical parameters of kidney in chickens[J]. Acta Veterinaria et Zootechnica Sinica, 2010, 41(1):86-91. (in Chinese)
[4] 张爱君. 砷中毒对抗氧化酶系统的影响[J]. 中国地方病防治杂志, 2014, 29(1):21-24.
ZHANG A J. Effects of arsenic poisoning on antioxidant enzyme system[J]. Chinese Journal of Control of Endemic Diseases, 2014, 29(1):21-24. (in Chinese)
[5] RADAK Z, ZHAO Z F, KOLTAI E, et al. Oxygen consumption and usage during physical exercise:the balance between oxidative stress and ROS-dependent adaptive signaling[J]. Antioxid Redox Signal, 2013, 18(10):1208-1246.
[6] LI W G, KONG A N. Molecular mechanisms of Nrf2-mediated antioxidant response[J]. Mol Carcinog, 2009, 48(2):91-104.
[7] 曹华斌, 苏荣胜,郭剑英,等. 不同来源日粮铜水平对肉鸡肝线粒体H₂O₂生成变化的研究[J]. 畜牧兽医学报, 2009, 40(12):1805-1811.
CAO H B, SU R S, GUO J Y, et al. Effects of dietary copper of different sources and dosages on hydrogen peroxide generation of mitochondria from broiler hepatocyte[J]. Acta Veterinaria et Zootechnica Sinica, 2009, 40(12):1805-1811. (in Chinese)
[8] 杨光,崔恒敏,邓俊良,等. 实验性雏鸡铜中毒的病理学研究[J]. 中国兽医科技, 2005, 35(10):801-806.
YANG G, CUI H M, DENG J L, et al. Pathological study of experimental copper toxicity in chickens[J]. Chinese Journal of Veterinary Science and Technology, 2005, 35(10):801-806. (in Chinese)
[9] 崔伟,彭西,赵丽,等. 高铜对雏鸭肾脏抗氧化功能的影响[J]. 畜牧兽医学报, 2009, 40(4):572-576.
CUI W, PENG X, ZHAO L, et al. Effect of high copper on the antioxydic function of kidney in ducklings[J]. Acta Veterinaria et Zootechnica Sinica, 2009, 40(4):572-576. (in Chinese)
[10] 刘好朋, 唐兆新,苏荣胜,等. 高铜日粮对肉鸡肝脏TrxR2基因mRNA表达和还原活性的影响[J]. 畜牧兽医学报, 2011, 42(3):423-428.
LIU H P, TANG Z X, SU R S, et al. Effect of high-level copper diet on expression of TrxR2 mRNA and reduction activity of TrxR2 of liver in broiler[J]. Acta Veterinaria et Zootechnica Sinica, 2011, 42(3):423-428. (in Chinese)
[11] DING H, ZHAO B. Change characteristics of women boxers' Na⁺, K⁺-ATPase, ROS and T-AOC before the match[J]. Energy Procedia, 2012, 17:671-677.
[12] 魏彦明, 李文广. 四君子汤体外抗脂质过氧化合活性氧自由基的作用[J]. 畜牧兽医学报, 2002, 33(2):197-199.
WEI Y M, LI W G. The effect of sijunzi decoction on lipid peroxidation and active oxygen free radicals in vitro[J]. Acta Veterinaria et Zootechnica Sinica, 2002, 33(2):197-199. (in Chinese)
[13] OLSON K R, GAO Y, ARIF F, et al. Metabolism of hydrogen sulfide (H₂S) and production of reactive sulfur species (RSS) by superoxide dismutase[J]. Redox Biol, 2018, 15:74-85.
[14] 生宝亮, 徐刚,陈德伟,等. 大鼠实验性高原肺水肿中T-AOC、MDA、SOD、CAT和IL-6的表达[J]. 第三军医大学学报, 2012, 34(23):2364-2367.
SHENG B L, XU G, CHEN D W, et al. T-AOC, MDA, SOD, CAT and IL-6 levels in rat pulmonary edema induced by hypobaric hypoxia[J]. Journal of Third Military Medical University, 2012, 34(23):2364-2367. (in Chinese)
[15] ZHOU Y H, YU J P, LIU Y F, et al. Effects of Ginkgo biloba extract on inflammatory mediators (SOD, MDA, TNF-α, NF-κBp65, IL-6) in TNBS-induced colitis in rats[J]. Mediators Inflamm, 2006, 2006:92642.
[16] 李素娟, 邢艳刚,薛星晨,等. 氟对小鼠睾丸谷胱甘肽抗氧化系统的影响[J]. 畜牧兽医学报, 2017, 48(5):954-962.
LI S J, XING Y G, XUE X C, et al. Effect of fluoride on glutathione antioxidant system in mice testis[J]. Acta Veterinaria et Zootechnica Sinica, 2017, 48(5):954-962. (in Chinese)
[17] 刘好朋, 贾雪霞,潘家强,等. 高铜日粮对肉鸡血清抗氧化功能的影响[J]. 黑龙江畜牧兽医, 2011(11):1-4.
LIU H P, JIA X X, PAN J Q, et al. Effect of different levels of copper sulfate in diet on serum antioxidant index in broilers[J]. Heilongjiang Animal Science and Veterinary Medicine, 2011(11):1-4. (in Chinese)
[18] ZHANG C, LIN J, GE J, et al. Selenium triggers Nrf2-mediated protection against cadmium-induced chicken hepatocyte autophagy and apoptosis[J]. Toxicol Vitro, 2017, 44:349-356.
[19] LINKER R A, LEE D H, RYAN S, et al. Fumaric acid esters exert neuroprotective effects in neuroinflammation via activation of the Nrf2 antioxidant pathway[J]. Brain, 2011, 134(3):678-692.
[20] YANG S H, LONG M, YU L H, et al. Sulforaphane prevents testicular damage in kunming mice exposed to cadmium via activation of Nrf2/ARE signaling pathways[J]. Int J Mol Sci, 2016, 17(10):1703.
[21] 梁小明, 邵天伟. 胆红素对机体的病理损伤和生理保护作用[J]. 实用医院临床杂志, 2012, 9(3):172-175.
LIANG X M, SHAO T W. Pathological damage and physiological protection of bilirubin in human body[J]. Practical Journal of Clinical Medicine, 2012, 9(3):172-175. (in Chinese)
[22] LIU J, WANG L, TIAN X Y, et al. Unconjugated bilirubin mediates heme oxygenase-1-induced vascular benefits in diabetic mice[J]. Diabetes, 2015, 64(5):1564-1575.
[23] REN J W, FAN C C, CHEN N, et al. Resveratrol pretreatment attenuates cerebral ischemic injury by upregulating expression of transcription factor Nrf2 and HO-1 in rats[J]. Neurochem Res, 2011, 36(12):2352-2362.
[24] KEUM Y S, HAN Y H, LIEW C, et al. Induction of heme oxygenase-1(HO-1) and NAD[P]H:quinone oxidoreductase 1(NQO1) by a phenolic antioxidant, butylated hydroxyanisole (BHA) and its metabolite, tert-butylhydroquinone (tBHQ) in primary-cultured human and rat hepatocytes[J]. Pharm Res, 2006, 23(11):2586-2594.
[25] WI?NIEWSKI M, LAPI?SKI M, ZDZIARSKA A, et al. Molecular cloning and analysis of Ancylostoma ceylanicum glutamate-cysteine ligase[J]. Mol Biochem Parasitol, 2014, 196(1):12-20.
[26] 吕航,朱佳玉,陈承杰,等. 急性亚砷酸钠暴露对小鼠主动脉Nrf2通路的影响[J]. 环境与健康杂志, 2017, 34(1):1-4.
LÜ H, ZHU J Y, CHEN C J, et al. Effects of acute sodium arsenite exposure on Nrf2 pathway in arota of mice[J]. Journal of Environment and Health, 2017, 34(1):1-4. (in Chinese)

The Effect of High Dietary Copper on Oxidative Damage and Expression of Nrf2 Signaling Pathway Related Genes in the Kidneys of Broilers

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